Process for producing cytotoxic lymphocytes

Details Process for producing cytotoxic lymphocytes Process for producing cytotoxic lymphocytes

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01) C12N 5/078 (2010.01) A61K 35/26 (2006.01) A61P 35/00 (2006.01) A61P 37/04 (2006.01) C07K 14/78 (2006.01) C12N 15/12 (2006.01)

Patent

CA 2536492

It is intended to provide a process for producing cytotoxic lymphocytes characterized by involving the step wherein, by using a medium having a total concentration of serum and plasma in the medium of from 0% by volume and less than 5% by volume, at least one treatment selected from among the induction of cytotoxic lymphocytes, maintenance thereof and upscaled culture thereof is carried out in the presence of fibronectin, its fragment or a mixture of the same.

L'invention concerne un procédé de production de lymphocytes cytotoxiques consistant à réaliser, au moyen d'un milieu présentant une concentration totale de sérum et de plasma comprise entre 0 % en volume et moins de 5 % en volume, au moins un traitement choisi parmi l'induction de lymphocytes cytotoxiques, leur entretien et leur culture à grande échelle, en présence d'une fibronectine, de son fragment ou d'un mélange correspondant.

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