Process for producing des(64,65)-proinsulin

Details Process for producing des(64,65)-proinsulin Process for producing des(64,65)-proinsulin

C - Chemistry, Metallurgy

07

K

530/3.1, 530/7.4

C07K 14/62 (2006.01) C07K 1/12 (2006.01)

Patent

CA 2031390

X-8017 Abstract of the Disclosure This application describes a process for pro- ducing des(64,65)-human proinsulin from human proinsulin by treating the latter with trypsin and carboxypeptidase B. The process is conducted by applying any or all of the following parameters: (1) use of an alkylated or acylated trypsin; (2) a temperature of from about 0°C. to about 10°C; (3) a pH of from about 8 to about 10; and (4) use of a buffer selected form the group consisting of ammon- ium bicarbonate and glycine.

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