Process for producing l-lysine by fermentation

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/60 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01) C12N 9/06 (2006.01) C12N 9/10 (2006.01) C12N 9/12 (2006.01) C12N 9/88 (2006.01) C12P 13/08 (2006.01)

Patent

CA 2178589

A bacterium belonging to the genus Escherichia, which is transformed by introducing, into its cells, a DNA coding for a dihydrodipicolinate synthase originating from a bacterium belonging to the genus Escherichia having mutation-to desensitize feedback inhibition by L-lysine and a DNA coding for an aspartokinase III originating from a bacterium belonging to the genus Escherichia having mutation to desensitize feedback inhibition by L-lysine; preferably a bacterium belonging to the genus Escherichia in which a dihydrodipicolinate reductase gene and a diaminopimelate dehydrogenase gene originating from Brevibacterium lactofermentum (or a succinyldiaminopimelate transaminase gene and a succinyldiaminopimelate deacylase gene) are further enhanced, is cultivated in an appropriate medium, L-lysine is produced and accumulated in a culture thereof, and L-lysine is collected from the culture.

Procédé de production de L-lysine consistant à: faire la culture, dans un milieu approprié, d'une bactérie Escherichia transformée par l'introduction dans ses cellules d'un ADN codant pour une dihydrodipicolinate synthase dérivée d'Escherichia, présentant une telle variation qu'elle peut être libérée à partir de la rétroinhibition causée par la L-lysine, et d'un ADN codant pour une aspartokinase III dérivée d'Escherichia présentant une variation telle qu'elle peut être libérée à partir de la rétroinhibition causée par la L-lysine, la bactérie Escherichia susmentionnée ayant été, de préférence, enrichie au moyen d'un gène de dihydrodipicolinate réductase et d'un gène de diaminopimelate deshydrogénase dérivé d'une bactérie du genre Corynebacterium (ou bien un gène de succinyldiaminopimelate transaminase et un gène de succinyldiaminopimelate deacylase); laisser accumuler la L-lysine produite dans le milieu de culture; et récolter la L-lysine accumulée dans le mileu de culture.

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