Process for producing l-phenyl acetyl carbinol (pac),...

Details Process for producing l-phenyl acetyl carbinol (pac),... Process for producing l-phenyl acetyl carbinol (pac),...

C - Chemistry, Metallurgy

12

N

195/86, 195/34.9

C12N 1/00 (2006.01) C12P 7/00 (2006.01) C12P 7/26 (2006.01)

Patent

CA 2000720

ABSTRACT Mutations are induced in a microorganism selected from the species Saccharomyces cerevisiae or from the species Candida flateri. The resulting mutants are cultured in the presence of a fermentation inhibitor, such as acetaldehyde, ephedrine or PAC- dione, to form colonies having resistance to the inhibitor. Cells from the colonies are isolated and tested for yield of phenyl acetyl carbinol (PAC) in a fermentation with benzaldehyde and pyruvate. Yeast cells from the colonies that produce ele- vated levels of PAC are selected for use in subsequent fermenta- tions. PAC is useful as an intermediate in the preparation of 1- ephedrine and d-pseudoephedrine, two well-known medicinal chemicals. -32-

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