Process for producing lactoperoxidase

Details Process for producing lactoperoxidase Process for producing lactoperoxidase

C - Chemistry, Metallurgy

12

N

C12N 9/04 (2006.01) B01D 15/04 (2006.01) B01D 61/14 (2006.01) B01J 39/04 (2006.01) B01J 49/00 (2006.01)

Patent

CA 2553733

A process for producing lactoperoxidase, including: a step (1) for bringing one or more milk materials into contact with a cation exchanger having weakly acidic groups as ion exchange groups to thereby effect adsorption treatment; a step (2) for washing the cation exchanger after the adsorption treatment; a step (3) for bringing the cation exchanger after the washing into contact with a leaching solvent to thereby obtain a leaching solution having lactoperoxidase eluted into the leaching solvent; a step (4) for concentrating the leaching solution through an ultrafiltration membrane to thereby effect precipitation in the concentrated leaching solution; and a step (5) for obtaining a lactoperoxidase solution by removing the precipitation from the concentrated leaching solution.

Procédé pour produire de la lactoperoxydase, comprenant les étapes consistant à (1) mettre un lait brut en contact avec un échangeur de cations ayant des groupes acides faibles comme groupes d'échange d'ions pour effectuer de cette manière un traitement d'adsorption ; (2) laver l'échangeur de cations après le traitement d'adsorption ; (3) mettre l'échangeur de cations après le lavage en contact avec un solvant éluant pour obtenir de cette manière une solution d'élution ayant de la lactoperoxydase dissoute dans le solvant d'élution ; (4) concentrer la solution d'élution par une membrane d'ultrafiltration pour effectuer de cette manière une précipitation dans la solution d'élution concentrée ; et (5) obtenir une solution de lactoperoxydase en enlevant les précipités de la solution d'élution concentrée.

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