Process for producing ligand binding protein with the use of...

Details Process for producing ligand binding protein with the use of... Process for producing ligand binding protein with the use of...

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01) C12P 21/02 (2006.01) C12Q 1/00 (2006.01) G01N 33/15 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2499644

It is intended to provide a process for efficiently producing a ligand binding protein characterized in that this protein or its domain is synthesized in a cell-free protein synthesis system containing a ligand binding to the protein or its domain. In a preferred embodiment, the above-described ligand is a substance selected from the group consisting of DNAs, RNAs, nucleosides, nucleotides, proteins, peptides, amino acids, lipids, saccharides, derivatives thereof, alkaloids, terpenes, low-molecular weight compounds, coenzymes, low- molecular weight metal complexes and metal ions and the ligand binding protein or the ligand binding domain thereof as described above is synthesized as a soluble protein.

L'invention concerne un procédé de production efficace d'une protéine de liaison à un ligand caractérisé en ce que cette protéine, ou en ce que son domaine, est synthétisée dans un système de synthèse de protéine hors-cellule contenant un ligand se liant à la protéine ou à son domaine. Dans un mode de réalisation préféré, le ligand décrit ci-dessus est une substance sélectionnée dans le groupe comprenant des ADN, ARN, nucléosides, saccharides, leurs dérivés, alcaloïdes, terpènes, composés de faible poids moléculaire, coenzymes, complexes métalliques de faible poids moléculaire et ions métalliques, et la protéine de liaison au ligand, ou le domaine de liaison au ligand, telle que décrite ci-dessus, est synthétisée sous forme de protéine soluble.

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