Process for producing microbial fat or oil having lowered...

C - Chemistry – Metallurgy – 12 – P

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C12P 7/64 (2006.01) A23D 9/007 (2006.01) A23D 9/02 (2006.01)

Patent

CA 2501880

A process for producing a crude oil having lowered unsaponifiable matter content and/or ester-type sterol content and comprising a highly unsaturated fatty acid as a constituent fatty acid, characterized in that a microorganism capable of producing a fat or oil comprising an unsaturated fatty acid as a constituent fatty acid is cultured in a medium containing a nitrogen source concentration of 2 to 15% within a culture tank equipped with an agitation impeller satisfying the requirement that the ratio of the diameter of agitation impeller (= d) to the inner diameter of the culture tank (= D) is d/D = 0.30 to 0.6.

L'invention concerne un procédé de production d'une huile brute possédant une teneur en insaponifiable et/ou en stérol de type ester réduites et comprenant des acides gras hautement insaturés en tant que constituant de l'acide gras, caractérisé en ce qu'un micro-organisme capable de produire un gras ou une huile comprenant un acide gras insaturé en tant que constituant d'un acide gras est cultivé dans un milieu contenant une concentration source d'azote de 2 à 15 % dans un réservoir de culture équipé d'un agitateur satisfaisant la nécessité suivante : le rapport du diamètre de l'agitateur (= d) et le diamètre intérieur du réservoir de culture (= D) équivalent à d/D=0,30 à 0,6.

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