C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/09 (2006.01) C12N 9/10 (2006.01) C12N 9/52 (2006.01)
Patent
CA 2518049
It is intended to provide an actinomyces-origin neutral metalloprotease, which selectively cleaves the pro-structure moiety of a microorganism-origin protransglutaminase, and a gene encoding the same. An activated microorganism- origin transglutaminase from which the pro-structural moiety has been excised can be produced by culturing a microorganism having the gene encoding the above-described actinomyces-origin neutral metalloprotease transferred thereinto, allowing the microorganism to produce the actinomyces-origin neutral metalloprotease and then treating the microorganism-origin protransglutaminase therewith.
L'invention concerne une métalloprotéase neutre issue d'actinomyces, permettant de couper le fragment pro-structural d'une protransglutaminase issue d'un micro-organisme, et un gène la codant. Une transglutaminase issue d'un micro-organisme activée, dont le fragment pro-structural a été excisé, peut être produite selon un procédé consistant à cultiver un micro-organisme à l'intérieur duquel le gène codant pour ladite métalloprotéase neutre issue d'actinomyces a été transféré, ce qui permet au micro-organisme de produire la métalloprotéase neutre issue d'actinomyces, puis à traiter la protransglutaminase issue du microorganisme avec celle-ci.
Date Masayo
Kikuchi Yoshimi
Onishi Norimasa
Umezawa Yukiko
Yokoyama Keiichi
Ajinomoto Co. Inc.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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