Process for producing microbial transglutaminase

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) C12N 9/10 (2006.01)

Patent

CA 2237041

Disclosed are a protein having a transglutaminase activity, which comprises a sequence ranging from serine residue at the second position to proline residue at the 331st position in an amino acid sequence represented by SEQ ID No. 1 wherein the N-terminal amino acid of the protein corresponds to serine residue at the second position of SEQ ID No. 1, a DNA encoding the protein, a transformant having the DNA, and a process for producing a protein having a transglutaminase activity, which comprises the steps of culturing the transformant in a medium. The protein can be produced in a large amount with the transformant using a host such as E. coli.

Le brevet décrit une protéine ayant une activité transglutaminase, qui contient une séquence allant d'un résidu de sérine en deuxième position à un résidu de proline en position 331 dans une séquence d'acides aminés représentée par SEQ ID no 1, où l'acide aminé en position N-terminale de la protéine correspond au résidu de sérine en deuxième position de SEQ ID no 1, ainsi qu'un ADN codant la protéine, un transformant porteur de l'ADN et un mode de production d'une protéine ayant une activité transglutaminase, qui comprend les étapes de culture du transformant dans un milieu. La protéine peut être produite en grande quantité avec le transformant en utilisant un hôte comme E. coli.

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