Process for producing peptide

Details Process for producing peptide Process for producing peptide

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/58 (2006.01) C07K 14/585 (2006.01) C12N 9/38 (2006.01)

Patent

CA 2076320

A process for production of peptide (target peptide) comprising: A) culturing host cells transformed with plasmid able to express a gene coding for a fusion protein represented in the following formula: A-L-B wherein, B is a target peptide, A is a protective peptide which is a fragment of 90-210 amino acids from the N-terminal of E. coli-.beta.-galactosidase polypeptide, and L is a linker peptide positioned between the C-terminal of said protective peptide and the N- terminal of said target peptide and selected so that said fusion protein is treated by an enzyme or chemical substance, the above-mentioned target peptide is separated, and said linker peptide has basic amino acid residues selected so that the isoelectric point of the entire fusion protein A-L-B is adjusted to a range between 4.9 and 6.9; B) obtaining an insoluble fraction comprising inclusion bodies by homogenization of the cultured cells of said transform; C) solubilizing a fusion protein in said inclusion bodies by treatment of said insoluble fraction with solubilizing agent; and D) cleaving the peptide bond between the C-terminal of the linker amino acid residue and the N- terminal of the target peptide of said solubilized fusion protein to release said target peptide. The present invention is able to express a target peptide in a large amount and accumulate said target peptide in host cells in the form of inclusion bodies.

Affiliated with

Also associated with

Rating

Process for producing peptide does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for producing peptide, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for producing peptide will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-2078301