Process for producing peptides by using in vitro...

C - Chemistry – Metallurgy – 12 – P

Patent

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C12P 21/02 (2006.01) C07K 1/00 (2006.01)

Patent

CA 2400735

It is intended to construct a reaction system whereby a peptide produced in an in vitro peptide synthesis system can be efficiently isolated at a high purity from the reaction system, and, at the same time, to resolve the problem of the consumption of energy in the reaction system. A process for producing a peptide or a peptide derivative by using a reaction system of transcribing a DNA into an RNA and then translating the RNA produced or a reaction system of translating an RNA in vitro characterized in that a part or all of protein components constituting the transcription/ translation reaction system are labeled with one of a pair of substances adhering to each other and the other substance is used as an adsorbent for capturing said labeled protein components after translating.

L'invention est destinée à permettre l'élaboration d'un système de réaction dans lequel un peptide produit dans un système de synthèse peptidique in vitro peut être efficacement isolé, à un degré de pureté élevé, à partir du système de réaction, tout en résolvant le problème de consommation d'énergie du système de réaction. L'invention concerne un procédé de production d'un peptide ou d'un dérivé de peptide au moyen d'un système de réaction permettant de transcrire un ADN en ARN, puis de traduire l'ARN ainsi obtenu, ou au moyen d'un système de réaction permettant de transcrire un ARN in vitro caractérisé en ce qu'une partie ou l'intégralité des constituants protéiques constituant le système de réaction de transcription/traduction sont étiquetés avec l'une de deux paires de substances adhérant l'une à l'autre tandis que l'autre substance sert d'adsorbant pour capturer lesdits constituants protéiques étiquetés une fois la traduction effectuée.

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