C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/00 (2006.01) C07K 1/02 (2006.01) C12N 15/09 (2006.01)
Patent
CA 2354534
A process for producing a polypeptide in a cell-free protein synthesis system with the use of dialysis, which comprises forming the polypeptide via the translation or transcription/translation of a nucleic acid encoding the polypeptide in the cell-free protein synthesis system containing a concentrated cell extract, and then taking up the polypeptide. By using this process, a desired polypeptide can be synthesized within a short period of time at a high yield and at a low cost, compared with the conventional processes.
L'invention concerne un procédé permettant de produire un polypeptide dans un système de synthèse protéique acellulaire, notamment grâce à l'utilisation de la dialyse. Ce procédé consiste tout d'abord à former le polypeptide par traduction ou transcription/traduction d'un acide nucléique codant pour ce polypeptide dans le système de synthèse protéique acellulaire, cet acide nucléique contenant un extrait cellulaire concentré, puis à reprendre ledit polypeptide. En employant ce procédé, on peut synthétiser le polypeptide souhaité dans un laps de temps limité, et ce pour un rendement plus élevé et des coûts inférieurs à ceux des procédés traditionnels.
Kigawa Takanori
Yabuki Takashi
Yokoyama Shigeyuki
Riken
Smart & Biggar
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