Process for producing protein

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/75 (2006.01) C07K 14/32 (2006.01) C07K 14/485 (2006.01) C07K 14/62 (2006.01) C12N 9/28 (2006.01) C12N 15/11 (2006.01) C12N 15/31 (2006.01) C12N 15/56 (2006.01) C12N 15/62 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2082637

abstract A gene coding for a protein BBRP42 which is secreted at the initial stage of cultivation is cloned from the gene library of Bacillus brevis, then an expression unit vector containing a DNA coding for the signal peptide and a DNA coding for translation regulating signals is constructed. The process of cultivation of the host cell having a recombinant plasmid which is linked with the foreign gene at the downstream of the region coding for the signal peptide of this expression vector successfully leads to the secretion of the foreign genetic products from the host cell at the initial stage of cultivation where the proteolytic enzymes have not been secreted too much, and leads to efficient production of the foreign genetic products.

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