Process for producing protein in cell-free protein synthesis...

Details Process for producing protein in cell-free protein synthesis... Process for producing protein in cell-free protein synthesis...

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01) C07K 14/705 (2006.01) C07K 19/00 (2006.01) C12N 9/02 (2006.01) C12N 15/62 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2486913

A fused protein of thioredoxin with a target protein is expressed in a cell- free protein synthesis system to thereby synthesize at least a part of the above-described fused protein as a soluble protein. It is preferable that the target protein is a highly hydrophobic protein such as a membrane protein. In particular, a membrane protein such a G protein-coupled receptor can be produced in a state being highly soluble and capable of forming a biologically active stereostructure. Also, a recombinant vector for producing such a membrane protein in a cell-free protein synthesis system is provided.

Une protéine fusionnée de thiorédoxine avec une protéine cible est exprimée dans un système de synthèse de protéine sans cellule afin de synthétiser ainsi au moins une partie de la protéine fusionnée ci-dessus décrite sous la forme d'une protéine soluble. Il est préférable que la protéine cible soit une protéine hautement hydrophobe telle qu'une protéine de membrane. En particulier, une protéine de membrane telle qu'un récepteur couplé à une protéine G peut être produite dans un état hautement soluble et capable de former une stéréostructure bioactive. L'invention concerne également un vecteur recombiné permettant la production d'une telle protéine de membrane dans un système de synthèse de protéine sans cellule.

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