Process for producing subculturable lymphokine- producing...

Details Process for producing subculturable lymphokine- producing... Process for producing subculturable lymphokine- producing...

C - Chemistry, Metallurgy

12

N

195/1.101

C12N 15/00 (2006.01) C07K 14/52 (2006.01) C07K 14/525 (2006.01) C12N 5/02 (2006.01) C12N 5/16 (2006.01) C12P 21/00 (2006.01)

Patent

CA 1202917

ABSTRACT A process for preparing human T cell hybridomas which are subculturable and produce lymphokines comprising the steps of 1) treating a human acute leukemia cell with a protein and/or RNA synthesis inhibitor; 2) independently activating a human T cell with a mitogen or antigen; 3) fusing the thus-treated human acute leukemia cell with the thus-activated human T cell in the presence of a fusion accelerator; and 4) isolating the thus-formed hybridoma.

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