C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 1/14 (2006.01) C07K 14/46 (2006.01) C12N 15/00 (2006.01)
Patent
CA 2369869
It is provided a method for producing a subunit peptide originating from an oligomeric protein having disulfide bonds within a subunit and between subunits. The method comprises producing a subunit originating from an oligomeric protein having disulfide bonds within a subunit and between subunits by the following steps: (a) a step of refolding the subunit by denaturing the oligomeric protein or its subunit in a solution with a protein-denaturing agent and removing the denaturing agent from the solution in the presence of polyoxyalkyl polyether having a functional group that reacts with a thiol group; and (b) a step of isolating the subunit bonded to the polyoxyalkyl polyether from the solution.
L'invention concerne un procédé de production d'une sous-unité de peptide issue d'une protéine de polymère qui possède des liaisons disulfure à l'intérieur d'une sous-unité et entre sous-unités. Le procédé comporte les étapes consistant à : (a) dénaturer la protéine de polymère ou sa sous-unité au moyen d'un agent dénaturant dans une solution, et éliminer ensuite l'agent dénaturant de la solution en présence d'un polyéther de polyoxyalkyle comportant un groupe fonctionnel réagissant avec un groupe thiol, ce qui permet de dérouler la sous-unité ; et (b) séparer la sous-unité, à laquelle le polyéther de polyoxyalkyle a été lié, de la solution.
Fukuchi Naoyuki
Kageyama Shunsuke
Kayahara Takashi
Kito Morikazu
Yamamoto Hiroshi
Ajinomoto Co. Inc.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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