Process for producing the a and b subunits of cholera toxin,...

C - Chemistry – Metallurgy – 12 – N

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195/131, 167/37,

C12N 15/00 (2006.01) C07K 14/28 (2006.01) C12P 21/02 (2006.01) G01N 33/569 (2006.01) A61K 39/00 (2006.01)

Patent

CA 1212640

ABSTRACT OF THE DISCLOSURE DNA sequences and recombinant DNA molecules com- prising at least a portion coding for all or part of subunits A and/or B of cholera toxin are prepared by enzymatic digestion of DNA of V. cholerae strains, iso- lating specific fragments and inserting them in appro- priate vectors and subunits A and/or B of cholera toxin are prepared by culture of microorganisms containing said modified vectors.

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