Process for producing the enzyme cholesterase and for...

C - Chemistry – Metallurgy – 12 – N

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195/123, 195/129

C12N 9/16 (2006.01) C12N 9/18 (2006.01) C12Q 1/60 (2006.01)

Patent

CA 1166986

ABSTRACT The enzyme cholesterase is produced by cultivating a strain of the Achromobacter delicatulus type, isolated from vegetable debris and identified by the symbol NRRL B-12115. Under the described culture conditions, the enzyme is exo- cellular and therefore does not require extraction from the cells. In addition, the micro organism has a duplication time less than that of moulds and Actinomycetes. Cholesterol esters of fatty acids can be hydrolysed by adding them to the culture medium be ore inoculating the strain.

382015

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