Process for producing thrombin

Details Process for producing thrombin Process for producing thrombin

C - Chemistry, Metallurgy

12

N

C12N 9/74 (2006.01)

Patent

CA 2190454

The present invention provides a production method of thrombin which can convert prothrombin into thrombin highly efficiently even in the absence of thromboplastin and blood plasma, easily obtain a starting material for use in the conversion of prothrombin into thrombin, and prepare and purify thrombin in an industrial scale. It is characterized in treating a prothrombin-containing aqueous solution with a Ca salt at 0 to 15°C.

Cette invention se rapporte à un procédé pour produire de la thrombine en traitant une solution aqueuse de prothrombine avec un sel de calcium à une température comprise entre 0 et 15 ~C. Dans ce procédé, la prothrombine peut être efficacement convertie en thrombine même en l'absence de thromboplastine et de plasma et la matière de départ pour cette conversion est facilement disponible. Grâce à ce procédé, de la thrombine peut être produite et purifiée à une échelle industrielle.

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