Process for producing trans-4-hydroxy-l-proline

Details Process for producing trans-4-hydroxy-l-proline Process for producing trans-4-hydroxy-l-proline

C - Chemistry, Metallurgy

12

N

C12N 9/02 (2006.01) C12P 13/24 (2006.01)

Patent

CA 2131775

Provided is a process for industrially producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L- proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium or Amycolatopsis and which catalyzes the hydroxylation of L- proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium. Also provided is a novel enzyme L-proline-4- hydroxylase, which is useful for the process.

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