Process for producing ubiquinone-10

C - Chemistry – Metallurgy – 12 – P

Patent

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C12P 23/00 (2006.01) C12N 1/21 (2006.01) C12N 9/10 (2006.01) C12N 15/00 (2006.01) C12N 15/54 (2006.01)

Patent

CA 2385132

The present invention provides a process for producing ubiquinone-10 using a microorganism having the ability to form ubiquinone-10 and having one or more properties selected from the group consisting of the property wherein geranylgeranyltransferase activity is reduced or defective, the property wherein decaprenyldiphosphate synthetase activity is strengthened, and the property wherein p- hydroxybenzoic acid-decaprenyltransferase activity is strengthened, DNA and a polypeptide useful for the production process, microorganisms useful for the production., a method for expressing a gene in the microorganisms, and a method for breeding the microorganisms.

Cette invention a trait à un procédé de production d'ubiquinone-10 à l'aide d'un micro-organisme capable de produire de l'ubiquinone-10 possédant une ou plusieurs caractéristiques sélectionnées parmi des caractéristiques faisant montre d'une activité de géranylgéranyl transférase réduite ou non, d'une activité renforcée de décaprényl diphosphate synthase ainsi que d'une activité renforcée d'hydrobenzoïque acide-décaprényl transférase. L'invention a également trait à un ADN, à un polypeptide ainsi qu'à un micro-organisme utilisés dans le cadre du procédé de production susmentionné. Elle porte, de surcroît, sur une technique d'expression génique dans le micro-organisme susmentionné ainsi que sur une technique d'amélioration génétique de ce micro-organisme.

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