Process for producing vaccine for bacterial toxin belonging...

Details Process for producing vaccine for bacterial toxin belonging... Process for producing vaccine for bacterial toxin belonging...

C - Chemistry, Metallurgy

12

P

C12P 21/00 (2006.01) A61K 39/02 (2006.01) C07K 1/30 (2006.01) C07K 14/195 (2006.01) C07K 14/285 (2006.01) C12N 9/14 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2109233

Bacteria to produce toxin belonging to RTX toxin family are proliferated in a nutrient enriched medium. A concentrated supernatant of the culture is added with a cationic surface agent to precipitate RTX toxin alone, not containing lipopolysaccharides and then the precipitate is washed by distilled water. Thus, lipopolysaccharides are efficiently removed from RTX toxin and a vaccine for RTX toxin family having high safety is prepared.

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