C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/071 (2010.01) C12N 5/077 (2010.01) A61K 31/70 (2006.01) A61K 35/12 (2006.01) A61K 48/00 (2006.01) A61P 1/00 (2006.01) A61P 1/16 (2006.01) A61P 1/18 (2006.01) A61P 5/00 (2006.01) A61P 7/00 (2006.01) A61P 9/00 (2006.01) A61P 11/00 (2006.01) A61P 11/02 (2006.01) A61P 13/00 (2006.01) A61P 13/12 (2006.01) A61P 15/00 (2006.01) A61P 17/00 (2006.01) A61P 19/00 (2006.01) A61P 21/00 (2006.01) A61P 25/00 (2006.01) A61P 27/02 (2006.01) A61P 35/00 (2006.01) A61P 35/02 (2006.01) A61P 37/00 (2006.01) C12N 5/10 (200
Patent
CA 2307108
Described is a process for generating a mammalian cell line from primary mammalian cells, comprising the step of: a) pre-treating a culture of said primary mammalian cells or a suspension thereof with at least one glucocorticoid, b) optional step comprising obtaining a suspension of said pre-treated culture of step a), c) transferring into the pre-treated cells of the suspension of step a) or b) at least one nucleic acid vector which is not of retroviral origin and which is competent to immortalize said pre-treated cells and d) culturing the transferred cells of step c). Furthermore, mammalian cell lines and cells obtainable by the described process are provided as well as pharmaceutical and diagnostic compositions containing such cells.
Braun Serge
Perraud Frederic
Ridout & Maybee Llp
Transgene S.a.
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