Process for production of peptidylglycine ó-hydroxylating...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/02 (2006.01) C07K 7/23 (2006.01) C07K 14/585 (2006.01) C07K 14/60 (2006.01) C12N 15/866 (2006.01) C12P 21/02 (2006.01) C12Q 1/26 (2006.01)

Patent

CA 2038569

4-17998/A/CGJ 43 Process for Production of Peptidyglycine .alpha.-hydroxylating Monooxygenase and Use thereof Abstract of the Disclosure A process for the production of peptidylglycine .alpha.-hydroxylating monooxygenase, comprising the steps of: culturing insect cells transfected with a recombinant baculovirus to which a DNA coding for the peptidylglycine .alpha.-hydroxylating monooxygenase has been incorporated to produce the enzyme, and recovering the enzyme from the culture; and a process for the production of a C-terminal .alpha.-hydroxyglycyl peptide, comprising converting a C-terminal glycine-extended peptide to a C-terminal .alpha.-hydroxyglycyl peptide using the enzyme prepared by the above process.

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