Process for production of sarcosine oxidase

C - Chemistry – Metallurgy – 12 – N

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195/34.8

C12N 9/06 (2006.01) C12Q 1/26 (2006.01)

Patent

CA 1112192

PROCESS FOR PRODUCTION OF SARCOSINE OXIDASE Abstract of the Disclosure An enzyme sarcosine oxidase is produced by culturing a micro- organism belonging to the genus Bacillus, and particularly the species Bacillus sp. B-0618 FERM-P No. 4049, and isolating the sarcosine oxidase thus produced from the culture medium. Sarcosine oxidase is useful for the determination of creatinine in the presence of creatinase and creatininase in a sample by mixing the enzymes with the sample and then measuring the generated hydrogen peroxide, formaldehyde or glycine.

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