Process for propagation and/or selection of plant material

Details Process for propagation and/or selection of plant material Process for propagation and/or selection of plant material

A - Human Necessities

01

H

A01H 5/00 (2006.01) A01H 4/00 (2006.01) A01H 7/00 (2006.01) C12N 5/04 (2006.01) C12N 15/84 (2006.01)

Patent

CA 2240847

A process for the micropropagation of shoots, rooted shoots or seedlings of a woody plant, which comprises cultivating the shoots, rooted shoots or seedlings in an oxygenated liquid culture medium, the shoots, rooted shoots or seedlings being submerged in the culture medium. They may move freely, for example, tumbling in the medium, or their movement may be restricted. The resulting plant material is obtained in high yields and has high quality. When genetically modified shoots, rooted shoots and seedlings have a selectable property, for example, resistance to a herbicide or antibiotic, they may be selected by submerged cultivation in an oxygenated liquid culture medium that comprises means for the selection, for example, the herbicide or antibiotic.

Ce procédé de micro-multiplication de pousses, pousses enracinées ou plants de semis d'un végétal ligneux, consiste à les cultiver dans un milieu de culture liquide oxygéné où il sont submergés et peuvent soit se déplacer librement, en s'y retournant par exemple, soit être fixés. Les plantes résultantes s'obtiennent avec des rendements et une qualité élevés. Quand des pousses, pousses enracinées ou plants génétiquement modifiés doivent présenter une caractéristique sélectionnée, la résistance à un herbicide ou à un antibiotique par exemple, cette sélection est possible par culture immergée dans un milieu de culture liquide oxygéné qui contient une substance de sélection, cet herbicide ou cet antibiotique par exemple.

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