Process for purification of antibodies

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 1/16 (2006.01)

Patent

CA 2726823

A method for the purification of a protein, in particular an 1g1v1 antibody, which comprises a chromatography step using a non-ionic polymer (such as hydroxyapatite chromatography and polyethylene glycol as the polymer) to remove protein aggregates, followed by an ion exchange chromatography step using a solubility enhancing additive such as a urea compound, an alkylene glycol or a zwitterion, especially glycine.

L'invention concerne un procédé de purification d'une protéine, en particulier un anticorps IgM, qui comprend une étape de chromatographie utilisant un polymère non ionique (tel qu'une chromatographie à hydroxyapatite et du polyéthylèneglycol en tant que polymère) pour supprimer des agrégats de protéine, ce qui est suivi par une étape de chromatographie à échange d'ions utilisant un additif renforçant la solubilité tel que un composé d'urée, un alkylèneglycol ou un zwitterion, en particulier de la glycine.

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