Process for purifying a protein

Details Process for purifying a protein Process for purifying a protein

C - Chemistry, Metallurgy

07

K

C07K 1/22 (2006.01) B01D 15/38 (2006.01) C07K 14/73 (2006.01) C12N 9/00 (2006.01)

Patent

CA 2050277

2050277 9012803 PCTABS00002 A process for the purification of proteins from solutions containing contaminants of similar net charge and molecular weight is provided, comprising contacting a solution containing the desired protein with an immobilized metal affinity chromatography resin in a buffer containing a low concentration of a weak ligand for the chelant of the resin. The adsorbed protein is then eluted using a buffer having a high concentration of the same weak ligand, e.g., Tris. Particularly preferred features employ agarose-iminodiacetic acid resins having copper cations and are especially useful in obtaining preparations of homogeneous, stable rsT4 proteins.

Affiliated with

Also associated with

Rating

Process for purifying a protein does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for purifying a protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for purifying a protein will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1661667