Process for purifying chemically synthesized rna

Details Process for purifying chemically synthesized rna Process for purifying chemically synthesized rna

C - Chemistry, Metallurgy

07

H

C07H 21/02 (2006.01) A61K 31/7088 (2006.01) A61K 31/7105 (2006.01) A61K 48/00 (2006.01) C07H 1/06 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2468048

Disclosed is a process for purifying chemically synthesized RNA having one or more chemical modifications, comprising: (a) loading the RNA onto an anion exchange high-performance liquid chromatography (HPLC) column; (b) eluting the RNA by passing a suitable buffer through the column to obtain an eluate; and (c) collecting the eluate from the column and recovering the RNA from the eluate, under conditions which allow for purification of the RNA. The RNA to be purified may be produced by deprotecting one or more protecting groups.

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