Process for purifying hepatitis b viral surface antigen...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/51 (2006.01) A61K 39/29 (2006.01) C07K 1/16 (2006.01) C07K 14/02 (2006.01) C12N 1/06 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2164672

A process for purifying hepatitis B viral surface antigen comprising the preS2 peptide from the cells of a recombinant organism is carried out by a sequence of steps which includes the step of disrupting the cells using a buffer containing a chaotropic salt to obtain a cell homogenate and the step of alkalifying the cell homogenate to a pH ranging from 11.0 to 13.5.

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