C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/78
C12N 9/72 (2006.01)
Patent
CA 1102263
ABSTRACT OF THE DISCLOSURE A process for purifying urokinase by contacting a crude urokinase-containing solution with a strongly acidic cation exchanger having a sulfonic acid group and adjusted to the NH4+ type to adsorb urokinase on said cation exchanger and then eluting urokinase from said exchanger, character- ized in that urea is previously added to the crude urokinase-containing solution in such a rate that the final concentration of urea in the solution is 3 to 8 M. This process is capable of providing purified urokinase having substantially constant and high specific activity from a crude urokinase-containing solution with a high recovery regardless of purity of the crude urokinase.
304573
Bai Yasuo
Ogino Shigeo
Yanagi Hideki
Yoshikawa Junichi
Marks & Clerk
Sumitomo Chemical Company Limited
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