Process for readily detecting or identifying one base alone...

C - Chemistry – Metallurgy – 12 – Q

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150/8.5

C12Q 1/68 (2006.01)

Patent

CA 2038932

2038932 9102087 PCTABS00104 Procédé de détection et/ou d'identification d'une seule base sur une séquence d'acide nucléique. Ce procédé est caractérisé en ce que: (1) on hybride la séquence sur laquelle se trouve la base à identifier avec un nucléotide de longueur suffisante; (2) on débute la synthèse du brin complémentaire de l'hybride obtenu en (1), -ledit nucléotide servant d'amorce- en présence: d'une polymérase sans action exonucléase 3'5' et d'au moins une base nucléotidique modifiée; (3) on détecte la base nucléotidique bloquante incorporée par tout moyen approprié. Application: diagnostic des maladies génétiques.

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