Process for recovering human ifn-.beta. from a transformed...

Details Process for recovering human ifn-.beta. from a transformed... Process for recovering human ifn-.beta. from a transformed...

C - Chemistry, Metallurgy

12

N

167/44, 195/1.3

C12N 15/00 (2006.01) C07K 14/565 (2006.01)

Patent

CA 1187013

Abstract A process for recovering IFN-.beta. from trans- formed bacteria comprising: disrupting the cell mem- branes of the bacteria solubilizing the IFN-.beta. from the disruptate into an aqueous medium with a solubili- zing agent such as sodium dodecyl sulfate, extracting the IFN-.beta. from the aqueous medium with 2-butanol, 2-methyl-butanol, or mixtures thereof under conditions that maintain phase separation between the aqueous medium and the extractant; and isolating the IFN-.beta. from the extractant such as by precipitating the IFN-.beta. from an aqueous buffer mixture of the extractant by lowering the pH thereof.

421837

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