Process for recovering microbially produced interferon-beta

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

530/13, 530/3.08

C07K 14/565 (2006.01) A61K 38/21 (2006.01) C07K 1/113 (2006.01)

Patent

CA 1340586

An approved procedure for the purification and renaturation of biologically active, bacterially produced IFN-beta is described. The partially purified material obtained by solubilization of isolated refractile bodies from the recombinant cells is treated to obtain reduction of the protein in the presence of a chaotropic environment and then oxidized after removal of the reducing agent. However, the chaotropic environment is retained during the oxidation. Upon removal of the chaotropic environment, a solubilizing additive is supplied to maintain the IFN-beta in solution. Further purification by conventional means may also be effected.

578236

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