Process for recovering peptides expressed as fusion proteins

Details Process for recovering peptides expressed as fusion proteins Process for recovering peptides expressed as fusion proteins

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 1/00 (2006.01) C07K 1/113 (2006.01) C07K 14/575 (2006.01) C07K 14/585 (2006.01) C07K 19/00 (2006.01) C12N 15/16 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2099967

ABSTRACT OF THE DISCLOSURE A process for recovering peptides which are expressed as fusion proteins comprises solubilizing the fusion protein and subjecting it to proteolytic cleavage, the improvement comprising citraconylating free amino groups prior to solubilization, thereby both to enhance solubility of the fusion protein in conditions conducive to proteolytic cleavage and to improve yield.

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