Process for recovering protease

Details Process for recovering protease Process for recovering protease

C - Chemistry, Metallurgy

12

P

195/128

C12P 21/00 (2006.01) C12N 9/50 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1100427

ABSTRACT OF THE DISCLOSURE Protease is recovered by reacting a first amino acid whose amino group is protected with a protective group with a second amino acid whose carboxyl group is protected with a pro- tective group, in an aqueous medium in the presence of a protease to result in peptide synthesis to deposit the addition compound of a dipeptide and said second C-terminal protected amino acid, said addition compound is dissolved by adding an organic solvent to a precipitate separated from the reaction mixture and isolating the protease from an organic solvent suspension or dissolving said addition compound by adding a water immiscible organic solvent and separating the organic solvent phase by a liquid-liquid separation. The addition compound can be dissolved in an organic solvent after separating the precipitate from the reaction mixture. The addition compound can be also dissolved by adding a water-immiscible organic solvent to the reaction mixture and the organic solvent phase can be separated from the aqueous phase.

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