C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
530/15.06, 167/1
C07K 14/765 (2006.01)
Patent
CA 1111023
ABSTRACT OF THE DISCLOSURE Purified albumin is recovered from human blood plasma without denaturing the globulins present therein, by mixing the blood plasma with 150 to 300 g polyethylene glycol (PEG) per liter of liquid mixture and adjusting the mixture to a pH of 6 to 7 for precipitating fibrinogen and globulins while forming a supernatant containing albumin. The supernatant is then separated from the formed precipitate, and the pH of the super- natant is adjusted to a pH of 4.5 to 5.3 for precipitating albumin. The albumin is redissolved with entrained PEG in an aqueous medium, the resulting albumin solution is subjected to thermocoagulation at 50 to 65°C and a pH of 4.6 to 5.0 in the presence of a stabilizing amount of caprylic acid, and the purified albumin is isolated from the solution by precipitation. This process enables to obtain a high yield of albumin with a high degree of purity, suitable for intravenous injection.
295364
Nordisk Insulinlaboratorium
Swabey Ogilvy Renault
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