C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) A61K 31/70 (2006.01) A61K 48/00 (2006.01) B01D 15/00 (2006.01) C12N 1/08 (2006.01)
Patent
CA 2206265
The invention relates to a process for reducing RNA concentration in a mixture of biological material, comprising the steps of: (a) providing a mixture of biological material having a first concentration of RNA; (b) filtering the mixture through a diatomaceous earth material to produce a filtrate having a second concentration of RNA, wherein the second concentration is less than the first concentration; and (c) collecting the filtrate having a reduced RNA concentration. In one aspect, the diatomaceous earth material serves to purify recombinant plasmid DNA from an RNA component in a mixture of biological material, and in another aspect, it serves to separate different forms of soluble RNA in a mixture of biological material.
L'invention se rapporte à un procédé de réduction de la concentration d'ARN dans un mélange de matières biologiques, ce procédé consistant à: (a) obtenir un mélange de matières biologiques possédant une première concentration d'ARN; (b) filtrer le mélange en le faisant passer dans une diatomite pour obtenir un filtrat possédant une seconde concentration d'ARN, cette seconde concentration étant inférieure à la première; et (c) récupérer le filtrat dont la concentration d'ARN a été réduite. Selon un aspect de l'invention, la diatomite sert à purifier l'ADN plasmidique de recombinaison à partir d'un composant d'ARN dans un mélange de matières biologiques, et selon un autre aspect, à séparer différentes formes d'ARN soluble dans un mélange de matières biologiques.
Budahazi Gregg
Horn Nancy
Marquet Magda
Meek Jennifer
Mbm Intellectual Property Law Llp
Vical Incorporated
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