Process for regenerating corn

Details Process for regenerating corn Process for regenerating corn

C - Chemistry, Metallurgy

12

N

47/4

C12N 5/04 (2006.01) A01H 1/02 (2006.01) A01H 4/00 (2006.01)

Patent

CA 1270648

-23- TITLE OF THE INVENTION PROCESS FOR REGENERATING CORN ABSTRACT OF THE DISCLOSURE The present invention relates to the regeneration of corn. The process comprises the steps of: (a) culturing tissue obtained from a corn plant on a first medium which comprises mineral salts, vitamins sucrose and a hormone for callus formation; (b) subculturing the calli on a second medium which comprises mineral salts, vitamins, sucrose and a hormone for callus maintenance; (c) subculturing the calli on a third medium which comprises mineral salts, vitamins, sucrose and optionally a hormone for shoot and root formation; and (d) optionally subculturing said shoots on a fourth medium which comprises mineral salts, vitamins, sucrose and optionally a hormone for plantlet maturation including additional root formation, whereby plants are obtained.

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