Process for regenerating corn

Details Process for regenerating corn Process for regenerating corn

A - Human Necessities

01

G

47/4

A01G 7/00 (2006.01) A01H 4/00 (2006.01)

Patent

CA 1269537

TITLE OF THE INVENTION: Process for Regenerating Corn ABSTRACT OF THE DISCLOSURE: The present invention relates to the regeneration of corn. The process comprises the steps of: (a) culturing tissue obtained from a corn plant on a first medium which comprises mineral salts, vitamins sucrose and a hormone in an amount sufficient to ensure callus formation (b) subculturing the calli on a second medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure callus maintenance; (c) subculturing the calli on a third medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure shoot and root formation; and (d) optionally subculturing said shoots on a fourth medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure plantlet maturation including additional root formation, whereby plants are obtained.

490131

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