Process for removing bacterial endotoxin from gram-negative...

Details Process for removing bacterial endotoxin from gram-negative... Process for removing bacterial endotoxin from gram-negative...

C - Chemistry, Metallurgy

08

B

167/185, 167/199

C08B 37/00 (2006.01) A61K 35/74 (2006.01) A61K 39/102 (2006.01)

Patent

CA 2018709

A process for removing endotoxin from Gram-negative polysaccharides such as polyribosylribitol phosphate by solubilizing polysaccharide-containing powder derived from Gram-negative bacteria fermentation broth to provide a divalent counter ion for endotoxin and adding alcohol incrementally to induce lipopolysaccharide precipition, and mixing material resulting from the alcohol addition with a nonionic resin, a detergent and a chelating agent. A process for removing endotoxin from Gram-negative polysaccharides such as H. influenzae polyribosylribitol phosphate by mixing with a nonionic resin, a detergent and a chelating agent. A process for removing endotoxin from Gram-negative polysaccharides such as H-influenzae polyribosylribitol phosphate by adding alcohol incrementally until substantially all lipopolysaccharide precipitates.

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