C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 1/14 (2006.01)
Patent
CA 2489773
The present invention is directed to a process for purifying alpha-i.PI. The comprises providing an impure protein fraction which comprises alpha-I-PL. The impure protein fraction is suspended in an aqueous solution at pH 6. Insoluble proteins are recovered and resuspended in aqueous solution at pH 8,5. PEG is added to precipitate .alpha.-2 proteins. To the PEG supernatant precipitation, which comprises alpha-1-PI, is added ZnCl2 to precipitate crude alpha-1-PI. The crude alpha-1-PI is resolubilized and applied to an anion-exchange medium. A fraction comprising alpha-1-PI is recovered from the anion-exchange medium. Alpha-1-PI purified by the process has a specific activity about 1.0 units/OD200. w
Hwang Duk Sung
Ito Hirokazu
Lepe Mark
Luz Lyndon
Nario Evelyn
Baxter Healthcare S.a.
Baxter International Inc.
Sim & Mcburney
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