C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/09 (2006.01) C07K 1/02 (2006.01) C07K 14/705 (2006.01) C12N 1/21 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2446031
In a process for producing a protein using a cell-free protein synthesis system, the system contains a surfactant so that the protein can be synthesized without agglutination. The above-described protein is a protein having a hydrophobic domain at least a part thereof, for example, a membrane protein or its fragment. The above-described surfactant is a mild surfactant which would not denature the protein, for example, a nonionic or amphoteric surfactant.
L'invention concerne une méthode de production de protéines qui met en oeuvre un système de synthèse de protéines acellulaire. Le système contient un tensioactif pouvant synthétiser la protéine sans agglutination. La protéine de l'invention présente un domaine hydrophobe ou au moins une partie dudit domaine, par exemple une protéine membranaire ou son fragment. Le tensioactif de l'invention est un tensioactif doux qui ne dénature pas la protéine, par exemple un tensioactif non ionique ou amphotère.
Ishihara Goushi
Kigawa Takanori
Shirouzu Mikako
Tajima Kaori
Yabuki Takashi
Riken
Smart & Biggar
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