Process for the activation of genetechnologically produced,...

Details Process for the activation of genetechnologically produced,... Process for the activation of genetechnologically produced,...

C - Chemistry, Metallurgy

07

K

530/13, 530/3.08

C07K 1/113 (2006.01) C07K 16/00 (2006.01) C12N 9/64 (2006.01) C12N 9/72 (2006.01)

Patent

CA 2000604

The present invention provides a process for the activation of gene-technologically produced, biologically active proteins expressed in prokaryotes after cell digestion by solubilisation under denaturing conditions and reducing conditions and subsequent reactivation under oxidising and renaturing conditions, wherein working is carried out at a protein concentration of 1 to 1000 µg./ml. and, between the solubilisation and the reactivation, a dialysis is carried out against a buffer with a pH value of from 1 to 4 containing 4 to 8 mole/litre guanidine hydrochloride or 6 to 10 mole/ litre urea.

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