C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/52 (2006.01) C12N 1/21 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 9/16 (2006.01) C12N 9/88 (2006.01) C12N 15/63 (2006.01) C12P 7/18 (2006.01)
Patent
CA 2733616
The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3--propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3--propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3- propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. Coli of a gene encoding a non-specific catalytic activity sufficient to convert 3--hydroxypropionaldehyde to 1,3-propanediol.
Emptage Mark
Haynie Sharon
Laffend Lisa
Pucci Jeff
Whited Greg
E.i. Du Pont de Nemours And Company
Genencor International Inc.
Torys Llp
LandOfFree
Process for the biological production of 1,3-propanediol... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for the biological production of 1,3-propanediol..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for the biological production of 1,3-propanediol... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1534104