Process for the biotechnological preparation of poly-...

Details Process for the biotechnological preparation of poly-... Process for the biotechnological preparation of poly-...

C - Chemistry, Metallurgy

12

P

195/131

C12P 7/62 (2006.01)

Patent

CA 1236415

ABSTRACT OF THE DISCLOSURE The present invention relates to a process for the bio- technological preparation of poly-D-(-)-3-hydroxybutyric acid (PHB) which comprises continuously culturing a microorganism that is a strain of Alcaligenes latus or a PHB-producing mutant thereof, in two separate successive fermentation stages with unrestricted supply of nutrients. The PHB-containing microorganism population cultured in the first stage with a dissolved oxygen content of 25 to 50% of the saturation value for air is then continuously transferred into a second fermentation stage in which the culture is continued at a dissolved oxygen content of 8 - 15% of the saturation value for air. The PHB is extracted in the usual manner from the biomass thereby obtained.

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