Process for the clostripain-catalyzed linkage of arg-pro and...

Details Process for the clostripain-catalyzed linkage of arg-pro and... Process for the clostripain-catalyzed linkage of arg-pro and...

C - Chemistry, Metallurgy

12

N

C12N 9/52 (2006.01) C07K 1/107 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2069199

ABSTRACT OF THE DISCLOSURE HOE 91/F 154 A process for the clostripain-catalyzed linkage of Arg- Pro and Arg-B (B = proteinogenous and non-proteinogenous amino acids) containing peptides The clostripain obtained from the culture filtrate from Clostridium histolyticum DSM 627 is employed for the linkage of arginine with amino-terminal protection or of peptides with amino-terminal protection and carboxyl- terminal arginine (acyl donor) to an amino acid or to a peptide with amino donor function. The enzyme can be obtained in high purity by alcohol precipitation and subsequent adsorption of the enzyme obtained from the alcohol-precipitated culture filtrate onto reactive dye and used for the linkage. The specific activity of clostripain is at least 80 U/mg. Clostripain catalyzes not only the abovementioned linkage of proteinogenous amino acids but also the linkage of a proteinogenous to a non-proteinogenous amino acid.

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