C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01)
Patent
CA 2474436
The present invention relates to the coarse clarification of lysed cell material from microoroganisms and particularly to a method of obtaining in nucleic acids. In certain embodiments, the present invention provides a method of coarsely clarifying cell lysate from microoganisms that comprises: lysing the microorganisms under the normal atmospheric pressure to form a flocculent precipitate in the lysate, compressing the flocculent precipitate in the lysate by reducing or increasing the pressure of the atmosphere surrounding the lysate in relation to the normal atmospheric pressure to form a compressed flocculent phase and a liquid phase, and separating the two phases.
L'invention concerne un procédé pour la purification grossière de lysats cellulaires de micro-organismes et, notamment, un procédé pour la préparation d'acides nucléiques.
Hucklenbroich Joerg
Mueller Markus
Fetherstonhaugh & Co.
Qiagen Gmbh
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