C - Chemistry – Metallurgy – 07 – D
Patent
C - Chemistry, Metallurgy
07
D
C07D 209/08 (2006.01) C07D 209/04 (2006.01) C07D 209/60 (2006.01) C07D 401/04 (2006.01) C07D 403/04 (2006.01) C07D 405/04 (2006.01) C07D 409/04 (2006.01) C07D 421/04 (2006.01) C07F 9/547 (2006.01) G01N 33/52 (2006.01)
Patent
CA 2139591
The method according to the invention consists in a) adding to the medium to be analyzed a compound of formula (I), A and C, which are the same or different, are each H, ( alpha ) or ( beta ) but not together H; and B= ( psi ), ( delta ), ( epsilon ), ( phi ), ( gamma ), ( eta ), ( iota , ( xi ) or ( kappa ); b) acidifying the medium, either with the aid of a strong carboxylic or sulfonic acid or perchloric acid, or with hydrochloric, hydrobromic or sulfuric acid; c) incubating the acidified medium between 25 and 60 DEG C to obtain a stable coloration; d) measuring the absorbency of the coloured medium at the absorption wavelength which is specific to the chromophoric additive formed and using the value of this measurement to determine the total concentration of 4-hydroxy-2-enaldehydes and MDA, or malonic dialdehyde only, according to the acid used. Application in the analysis of the indexes of lipid peroxidation, especially in clinical research.
2139591 9427155 PCTABS00168 Le procédé selon l'invention comprend: a) l'addition au milieu à analyser d'un composé de formule (I), A et C, identiques ou différents, représentent chacun H, (.alpha.) ou (.beta.), mais pas simultanément H; et B= (.psi.), (.delta.), (.epsilon.), (.phi.), (.gamma.), (.eta.), ($g(i)), ($g(j)) ou (.kappa.); b) l'acidification du milieu, soit par un acide carboxylique ou sulfonique fort ou l'acide perchlorique, soit par l'acide chlorhydrique, bromhydrique ou sulfurique; c) l'incubation du milieu acidifié entre 25 et 60 ·C, pour obtenir une coloration stable; d) la mesure de l'absorbance du milieu coloré à la longueur d'onde d'absorption qui est spécifique du produit d'addition chromophorique formé, et son utilisation pour déterminer la concentration totale en 4-hydroxy-2-énaldéhydes et en MDA, ou en dialdéhyde malonique seul, selon l'acide utilisé. Application au dosage des indices de peroxydation lipidique, notamment en recherche clinique.
Chaudiere Jean
Erdelmeier Irene
Gerard-Monnier Dominique
Yadan Jean-Claude
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Oxis Isle Of Man Limited
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