C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/131
C12P 7/56 (2006.01) C12P 1/00 (2006.01) C12P 7/42 (2006.01)
Patent
CA 1139696
ABSTRACT OF THE DISCLOSURE Water-soluble .alpha.-ketocarboxylic acids are continuously converted into the corresponding .alpha.-hydroxy carboxylic acids in a diaphragm reactor. The conversion is carried out in the presence of a substrate-specific dehydrogenase and, as the coenzyme, nicotinamide-adenine-dinucleotide (NAD+/NADH) which has been increased in its molecular weight by linkage to a water-soluble polymer having an average molecular weight between 500 and 50,000. At the same time NADH is continuously regenerated from NAD+ in the presence of a formate dehydrogenase and formate ions. The diaphragm must have an average diameter of 1 to 3 nm. The coenzyme is applied in a concentration of 0.1 to 10 millimoles per litre. The reactor is continuously fed with a substrate flow containing 50 to 100% of the maximally soluble amount but not more than 2,000 millimoles/litre of the .alpha.-keocarboxylic acid to be reacted, in the form of a water-soluble salt, and 100 to 6,000 millimoles/litre of a formate. A differential pressure of 0.1 to 15 bars is maintained across the diaphragm. A filtrate flow containing the .alpha.-hydroxy carboxylic acid formed is continuously drawn off behind the diaphragm.
356909
Buckmann Andreas
Kula Maria-Regina
Leuchtenberger Wolfgang
Wandrey Christian
Wichmann Rolf
Aktiengesellschaft Degussa
Gesellschaft Fur Biotechnologische Forschung Mbh (gbf)
Marks & Clerk
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